LOCUS       AB032966                4748 bp    mRNA    linear   PRI 10-MAY-2002
DEFINITION  Homo sapiens mRNA for KIAA1140 protein, partial cds.
ACCESSION   AB032966
VERSION     AB032966.2  GI:20521777
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Catarrhini;
            Hominidae; Homo.
REFERENCE   1
  AUTHORS   Hirosawa,M., Nagase,T., Ishikawa,K., Kikuno,R., Nomura,N. and
            Ohara,O.
  TITLE     Characterization of cDNA clones selected by the GeneMark analysis
            from size-fractionated cDNA libraries from human brain
  JOURNAL   DNA Res. 6 (5), 329-336 (1999)
  MEDLINE   20039618
   PUBMED   10574461
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Ohara,O., Nagase,T. and Kikuno,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-1999) Osamu Ohara, Kazusa DNA Research Institute,
            Laboratory of DNA Technology; 1532-3 Yana, Kisarazu, Chiba
            292-0812, Japan (E-mail:cdnainfo@kazusa.or.jp,
            URL:http://www.kazusa.or.jp/huge/, Tel:+81-438-52-3913,
            Fax:+81-438-52-3914)
COMMENT     On May 9, 2002 this sequence version replaced gi:6329944.
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="hk01004s1"
                     /sex="male"
                     /tissue_type="brain"
                     /clone_lib="pBluescriptII SK plus"
                     /dev_stage="adult"
                     /note="This sequence was obtained bysubcloning of the DNA
                     fragments derived from two cDNA clones (1 - 1023 was
                     derived from aj00420 and 1024 - 4748 was derived from
                     hk01004)."
     gene            1..4748
                     /gene="KIAA1140"
     CDS             <267..2525
                     /gene="KIAA1140"
                     /note="Start codon is not identified."
                     /codon_start=1
                     /product="KIAA1140 protein"
                     /protein_id="BAA86454.2"
                     /db_xref="GI:20521778"
                     /translation="SWANCITWRAHTEMPSACTHGPGLMTCPWRTSPCIRCGCCRRLL
                     SSKHASGFLGEHSPGGQRSCRGGLSLERLPNSIASRFRLTEREEEVITCFERASWIAQ
                     VFLQELEKTTNNSTSRHLKGCHPLDYELTYFLEAALQSAYVKNLKKGNIVKGMRELRE
                     VLRTVETKATQNFKVMAAKHLAGVLLHSLSEECYWSPLSHPLPEFMGKEESSFATQAL
                     RKPHLYEGDNLYCPKDNIEEALLLLLISESMATRDVVLSRVPEQEEDRTVSLQNAAAI
                     YDLLSITLGRRGQYVMLSECLERAMKFAFGEFHLWYQVALSMVACGKSAYAVSLLREC
                     VKLRPSDPTVPLMAAKVCIGSLRWLEEAEHFAMMVISLGEEAGEFLPKGYLALGLTYS
                     LQATDATLKSKQDELHRKALQTLERAQQLAPSDPQVILYVSLQLALVRQISSAMEQLQ
                     EALKVRKDDAHALHLLALLFSAQKHHQHALDVVNMAITEHPENFNLIFTKVKLEQVLK
                     GPEEALVTCRQVLRLWQTLYSFSQLGGLEKDGSFGEGLTMKKQSGMHLTLPDAHDADS
                     GSRRASSIAASRLEEAMSELTMPSSVLKQGPMQLWTTLEQIWLQAAELFMEQQHLKEA
                     GFCIQEAAGLFPTSHSVLYMRGRLAEVKGNLEEAKQLYKEALTVNPDGVRIMHSLGLM
                     LSRLGHKSLAQKVLRDAVERQSTCHEAWQGLGEVLQAQGQNEAAVDCFLTALELEASS
                     PVLPFSIIPREL"
ORIGIN      
        1 ccatgccaaa ataaaagact ccatgccttt gctggagaag aatgagccga agatgagcga
       61 agccaaaaat tatctaagca gtatccttaa ccatgggagg ctctcgggtc tcgctgtgtt
      121 actcaggctg gtctccaact cctgggctca agcgatcctc ctacttcagc ctcccgaagc
      181 gttgggatta caggagtgag gcactgcgcc cagctgattt ttaaagagct attccaaacc
      241 acagtacatg tgtgaggcca tgctgatcct gggcaaactg cattacgtgg agggctcata
      301 ccgagatgcc atcagcatgt acgcacgggc cgggattgat gacatgtcca tggagaacaa
      361 gcccctgtat cagatgcggc tgctgtcgga ggcttttgtc atcaaagcat gcctccggct
      421 tcttggggga acacagtccc ggtggtcaga ggtcctgcag gggaggcctc tctctggaac
      481 gcctacccaa ctccatcgcc tcccgcttcc gcctgacaga gagggaggag gaagtgatca
      541 cctgttttga gagggcctcc tggatcgctc aggtgttcct gcaggaattg gagaagacca
      601 caaataacag cacgtcgagg catctgaaag gctgtcaccc gcttgactat gagctcacct
      661 acttcctgga agctgccctc cagagcgcct atgtgaaaaa cctgaagaag gggaacatcg
      721 tgaagggcat gagagagctc cgggaggtgc tgcggactgt ggagaccaaa gcaactcaga
      781 acttcaaagt gatggcggcc aagcacctgg cgggggtcct gctgcactcc ctgagtgagg
      841 agtgctactg gagccccctg tcccaccctc tgcctgagtt catgggcaag gaggagagtt
      901 ctttcgccac tcaggccctg cggaaacctc acctctatga aggagacaac ctctactgcc
      961 ccaaggacaa catcgaggaa gccctcctgc tcctcctcat cagcgaatcc atggcaactc
     1021 gagatgtggt gctgagccgg gtgccggagc aggaggagga ccggacagtg agcttgcaga
     1081 atgccgcagc catctatgac ctcctgagca tcacgttggg cagaagggga cagtacgtca
     1141 tgctctcgga gtgcctggag cgagccatga agtttgcgtt tggagaattt cacctttggt
     1201 accaggtggc cctctccatg gtggcttgtg ggaagtcagc ctacgctgtg tccctgctgc
     1261 gggagtgtgt gaagttgcgg ccctcggacc ccaccgtgcc cctgatggcc gcgaaggtct
     1321 gcatcgggtc ccttcgctgg ctagaggaag cagagcactt tgccatgatg gtgatcagcc
     1381 tcggagagga agccggggag ttcctcccca agggctacct ggctctgggt ctcacctata
     1441 gcctgcaggc caccgacgcc accctgaagt ccaagcaaga tgaattgcac cggaaggcac
     1501 tgcagacgct ggagagggct cagcagctgg cgcccagtga cccccaggtc atcctctatg
     1561 tctcgctgca gctggccctc gtccgacaga tctccagtgc catggagcag ctgcaggagg
     1621 ccctgaaggt acgcaaggat gatgcccacg ccctccacct gctggcactg ctcttctctg
     1681 cccagaagca ccaccagcat gccctggatg ttgtcaacat ggccatcacc gagcaccctg
     1741 agaacttcaa cctgatcttc accaaggtga agctggagca ggtgctgaaa ggcccagagg
     1801 aagccctcgt gacctgcaga caagtgctga ggctgtggca gaccctgtac agcttctccc
     1861 agctgggagg cctagaaaag gatggcagct tcggtgaggg cctcaccatg aagaagcaga
     1921 gtggcatgca cctgactttg cctgatgccc atgatgcaga ctctggctcc cggcgggctt
     1981 cgtccatcgc cgcctcccgg ctggaggagg ccatgtcaga gctgactatg ccctcttcgg
     2041 tcctgaagca gggccccatg cagctgtgga ccacgctgga acagatctgg ctgcaggctg
     2101 ctgagctgtt catggagcag cagcacctca aggaagcagg tttctgcatc caggaggcgg
     2161 cgggcctctt ccccacttct cactcagtac tctatatgcg gggccggctg gctgaggtga
     2221 agggcaacct ggaggaggcc aagcagctgt acaaggaggc gctcacggtg aacccagatg
     2281 gcgtgcgcat catgcatagc ctgggtctga tgctgagtcg gctgggccac aagagcttgg
     2341 cccagaaggt gcttcgtgat gccgtggaga ggcagagtac gtgccacgag gcgtggcagg
     2401 gcctgggcga ggtgctgcag gcccagggcc agaacgaggc tgccgttgac tgcttcctca
     2461 ccgcccttga gctggaggcc agcagccctg tactgccctt ctccatcatc cccagagagc
     2521 tctgacgacg ctgcagccgc agggagggag gggctggcca gagggagagg cagcagggaa
     2581 cgtgggtcag ggtggggcaa cagtggcatc aggtgcgggg cctcagggaa atacatcttt
     2641 agtgaacgcc tctgcagctg cagccctcgt tctcttggct gggccaagag ggccttcctg
     2701 gatttctttg ttggtgcctt gggaaacagt ctgacttgaa ccctaagtgc ctttggagag
     2761 ttttgtggtg accagacttg ctccccaaga gctgggcagc ggggagcctc acagctgtcc
     2821 ttcaccctca cccatgcctc tggcttgggg tctgggtggg gggttctcac tccccactct
     2881 cagcacagta cagacttctg gatctctctc aggtcttgcc cagggcggtc acaatgtgaa
     2941 gaaactgcgg gcaagtggga agactatgag atttctgggt tcccttctca gacttggagt
     3001 tagtagatga ttcctgcatt gcccctgctt gccctctgag accagctggg ccccaccttg
     3061 ctctttcccc ctgctaccaa gtgcctttgg ggcctctggg gtctgaccag gggtactgag
     3121 caccggccct aacacttcca tctccaccca ccccatctcc ctggcgatgt gctccagccc
     3181 aagcagcctc cgtaggcttt agatcctgtg gttgctagat ccagtccttt ctaataccct
     3241 gagtcaacac attactcctg caggtcttag gctacaatgc aggtcccttg agggccacca
     3301 acatggaggt aggcagtttc taggactgtc cccagtacat ctcaccaccc acagcccttt
     3361 ttttgccttg attcgagcct caccctggcc ttttggcttc ccctgcctga gagagacctg
     3421 aggaggggac agagcccagc ccctctcctg tggctgagca ggcctctgtg tccatgacac
     3481 ctgtcttccg ggcctggggg ctgtgggtgt atgtcctccc tactggcttc cccggcccct
     3541 gctgcatgat gctcttggaa ctcttctcca aggagtcagt cccccaggcc tatcagggga
     3601 tccttttgta tctgcacttt gggttttagt ttcaaagctc catcaggtac agcttgcatt
     3661 tcaggatgtg tggaaagctc gggtgagggc tgccctggtt catcatagct ccaccttcct
     3721 cggaaggagt gggctgttgg agacccccca tccatggcac actagctcag cactgcattt
     3781 cccgagatga ttcccaagac agctggtgcc tcctggcttt cctgtgccag gccaaggggc
     3841 accacagagg accctggatc ctttgcctct tcttggttga aggatctcta tgtatgtgtg
     3901 tatataaata tagtttttta tctatatata taaaatagag atctattttt tttctggaat
     3961 tctgttagaa aagtaaagaa aaagcaaatg ctgttggttt atctcagggt gcccaaagtg
     4021 gttatagtca atttttggta ctaggaaagg cacccaatgc atttcctgac ttttaagcat
     4081 ttccttgttg gaagcagcag agggccaggc caagttgctg acagtgactt tgcaggttga
     4141 ataaagaaac ccttggaggg gaagcaggct tgtctgaagc agcatgtata ttcactgggc
     4201 atgtagctcc cacaccagcc ttgagccagg ccctggacag gaggggctgt tgcaggatga
     4261 gggaggccag agaaggcatc gaagccaaga cctgggccca gctggggagg gatgtgggaa
     4321 aggaaggatg ggagggagga ccctctggga aaatgtggat ttgagctggt gagagtgttg
     4381 ctaaggctgg gctaaagcct ggagagggta ggaggaggca agaggggtcc aggcagggct
     4441 gatcctggcc tctgacctgt ccagggcgac ccctgaagcc cctgctgcct ctgggcattg
     4501 ctgggagagg ccaaggcagg actcacgtct gaacagagat cccctcgggc attgctgatg
     4561 ggccaccttc agctgcaggg aagaagccta ggagaggagg catgggaggg acctgggcct
     4621 tgttcagatt ggccacctct gctgagaagt ccataccagt acacccctaa taagttatgc
     4681 cacataccaa cgtactgtgg atattataac ctgcattaaa acaactctaa agaacgctgc
     4741 tcatttac
//

LOCUS       NM_000075               1474 bp    mRNA    linear   PRI 27-OCT-2004
DEFINITION  Homo sapiens cyclin-dependent kinase 4 (CDK4), mRNA.
ACCESSION   NM_000075 NM_032913
VERSION     NM_000075.2  GI:16936531
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Wallace,M. and Ball,K.L.
  TITLE     Docking-dependent regulation of the Rb tumor suppressor protein by
            Cdk4
  JOURNAL   Mol. Cell. Biol. 24 (12), 5606-5619 (2004)
   PUBMED   15169919
  REMARK    GeneRIF: A novel Cdk4 docking motif has been defined within a
            stretch of 19 amino acids from the C-terminal domain of the Rb
            protein that are essential for Cdk4 binding.
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Liu,E., Li,X., Yan,F., Zhao,Q. and Wu,X.
  TITLE     Cyclin-dependent kinases phosphorylate human Cdt1 and induce its
            degradation
  JOURNAL   J. Biol. Chem. 279 (17), 17283-17288 (2004)
   PUBMED   15004027
  REMARK    GeneRIF: Cdk2 and Cdk4 phosphorylate human Cdt1 and induce its
            degradation
REFERENCE   3  (bases 1 to 1474)
  AUTHORS   Kahl,C.R. and Means,A.R.
  TITLE     Regulation of cyclin D1/Cdk4 complexes by
            calcium/calmodulin-dependent protein kinase I
  JOURNAL   J. Biol. Chem. 279 (15), 15411-15419 (2004)
   PUBMED   14754892
  REMARK    GeneRIF: cyclin D1/Cdk4 complexes are regulated by
            calcium/calmodulin-dependent protein kinase I
REFERENCE   4  (bases 1 to 1474)
  AUTHORS   Zhao,Q., Boschelli,F., Caplan,A.J. and Arndt,K.T.
  TITLE     Identification of a conserved sequence motif that promotes Cdc37
            and cyclin D1 binding to Cdk4
  JOURNAL   J. Biol. Chem. 279 (13), 12560-12564 (2004)
   PUBMED   14701845
  REMARK    GeneRIF: CDK4 binding to cyclin D1 is stimulated by Cdc37
REFERENCE   5  (bases 1 to 1474)
  AUTHORS   Miliani de Marval,P.L., Macias,E., Conti,C.J. and
            Rodriguez-Puebla,M.L.
  TITLE     Enhanced malignant tumorigenesis in Cdk4 transgenic mice
  JOURNAL   Oncogene 23 (10), 1863-1873 (2004)
   PUBMED   14647432
  REMARK    GeneRIF: susceptibility to skin tumor formation by forced
            expression of CDK4
REFERENCE   6  (bases 1 to 1474)
  AUTHORS   Klausen,P., Bjerregaard,M.D., Borregaard,N. and Cowland,J.B.
  TITLE     End-stage differentiation of neutrophil granulocytes in vivo is
            accompanied by up-regulation of p27kip1 and down-regulation of
            CDK2, CDK4, and CDK6
  JOURNAL   J. Leukoc. Biol. 75 (3), 569-578 (2004)
   PUBMED   14694185
  REMARK    GeneRIF: cyclin-dependent kinase (CDK)2, -4, and -6 were
            down-regulated from the myelocytes/metamyelocytes stages and onward
REFERENCE   7  (bases 1 to 1474)
  AUTHORS   Mishra,R. and Das,B.R.
  TITLE     Early overexpression of Cdk4 and possible role of KRF and c-myc in
            chewing tobacco mediated oral cancer development
  JOURNAL   Mol. Biol. Rep. 30 (4), 207-213 (2003)
   PUBMED   14672406
  REMARK    GeneRIF: Data suggest that cyclin dependent kinase 4 is involved in
            the development of tobacco-mediated oral carcinogenesis, and that
            c-myc expression is absent in normal and high in later stages of
            oral cancer development.
REFERENCE   8  (bases 1 to 1474)
  AUTHORS   Kim,D.M., Yang,K. and Yang,B.S.
  TITLE     Biochemical characterizations reveal different properties between
            CDK4/cyclin D1 and CDK2/cyclin A
  JOURNAL   Exp. Mol. Med. 35 (5), 421-430 (2003)
   PUBMED   14646596
  REMARK    GeneRIF: significant difference in their biochemical properties
            between CDK4/cyclin D1 and CDK2/cyclin A affecting regulation of
            cellular RB function
REFERENCE   9  (bases 1 to 1474)
  AUTHORS   Mori,N., Yang,R., Kawamata,N., Miller,C.W., Mizoguchi,H. and
            Koeffler,H.P.
  TITLE     Absence of R24C mutation of the CDK4 gene in leukemias and solid
            tumors
  JOURNAL   Int. J. Hematol. 77 (3), 259-262 (2003)
   PUBMED   12731669
  REMARK    GeneRIF: DNA mutation analysis of the CDK4 gene in many types of
            neoplasms reveal it is very rare. Random mutagenisis the CDK4 gene
            showed that most of the mutations that disrupted interactions with
            p16(INK4) also knocked out the activity of CDK4.
REFERENCE   10 (bases 1 to 1474)
  AUTHORS   Masaki,T., Shiratori,Y., Rengifo,W., Igarashi,K., Yamagata,M.,
            Kurokohchi,K., Uchida,N., Miyauchi,Y., Yoshiji,H., Watanabe,S.,
            Omata,M. and Kuriyama,S.
  TITLE     Cyclins and cyclin-dependent kinases: comparative study of
            hepatocellular carcinoma versus cirrhosis
  JOURNAL   Hepatology 37 (3), 534-543 (2003)
   PUBMED   12601350
  REMARK    GeneRIF: increases of cyclin D1, cyclin-dependent kinase 4, cyclin
            E, cyclin A, and Wee1 play an important role in the development of
            hepatocellular carcinoma from cirrhosis
REFERENCE   11 (bases 1 to 1474)
  AUTHORS   Gump,J., Stokoe,D. and McCormick,F.
  TITLE     Phosphorylation of p16INK4A correlates with Cdk4 association
  JOURNAL   J. Biol. Chem. 278 (9), 6619-6622 (2003)
   PUBMED   12529334
  REMARK    GeneRIF: Cdk4 binds to p16INK4A and causes its phosphorylation
REFERENCE   12 (bases 1 to 1474)
  AUTHORS   Ramirez,R.D., Herbert,B.S., Vaughan,M.B., Zou,Y., Gandia,K.,
            Morales,C.P., Wright,W.E. and Shay,J.W.
  TITLE     Bypass of telomere-dependent replicative senescence (M1) upon
            overexpression of Cdk4 in normal human epithelial cells
  JOURNAL   Oncogene 22 (3), 433-444 (2003)
   PUBMED   12545164
  REMARK    GeneRIF: Cdk4-overexpressing cells divide in an apparently normal
            regulated fashion, are able to respond to changes in calcium
            levels, and fully differentiate . These results suggest that the
            differentiation pathways in Cdk4-overexpressing cells remain intact
REFERENCE   13 (bases 1 to 1474)
  AUTHORS   Detjen,K.M., Murphy,D., Welzel,M., Farwig,K., Wiedenmann,B. and
            Rosewicz,S.
  TITLE     Downregulation of p21(waf/cip-1) mediates apoptosis of human
            hepatocellular carcinoma cells in response to interferon-gamma
  JOURNAL   Exp. Cell Res. 282 (2), 78-89 (2003)
   PUBMED   12531694
  REMARK    GeneRIF: Interferon gamma reduces the activity of Cdk4 and Cdk2,
            inhibiting he G1 cell cycle in human hepatocellular carcinoma
            cells.
REFERENCE   14 (bases 1 to 1474)
  AUTHORS   Katayama,K., Dobashi,Y., Kitagawa,M., Kawai,M., Kadoya,Y. and
            Kameya,T.
  TITLE     Cdk4/cyclin D1 kinase, a universal and critical regulator of
            apoptosis
  JOURNAL   Anticancer Res. 23 (1A), 235-243 (2003)
   PUBMED   12680219
  REMARK    GeneRIF: Cells overexpressing cdk4 or cyclin D1 exhibited nuclear
            features characteristic of apoptosis.
REFERENCE   15 (bases 1 to 1474)
  AUTHORS   Nikitakis,N.G., Drachenberg,C.B. and Papadimitriou,J.C.
  TITLE     MDM2 and CDK4 expression in carcinosarcoma of the esophagus:
            comparison with squamous cell carcinoma and review of the
            literature
  JOURNAL   Exp. Mol. Pathol. 73 (3), 198-208 (2002)
   PUBMED   12565795
  REMARK    GeneRIF: Data provide evidence of a role for MDM2 and CDK4 in the
            pathogenesis of carcinosarcoma.
REFERENCE   16 (bases 1 to 1474)
  AUTHORS   Lazarov,M., Kubo,Y., Cai,T., Dajee,M., Tarutani,M., Lin,Q.,
            Fang,M., Tao,S., Green,C.L. and Khavari,P.A.
  TITLE     CDK4 coexpression with Ras generates malignant human epidermal
            tumorigenesis
  JOURNAL   Nat. Med. 8 (10), 1105-1114 (2002)
   PUBMED   12357246
  REMARK    GeneRIF: data identify a new role for oncogenic Ras in CDK4
            regulation and highlight the functional importance of CDK4
            suppression in preventing uncontrolled growth
REFERENCE   17 (bases 1 to 1474)
  AUTHORS   Rheinwald,J.G., Hahn,W.C., Ramsey,M.R., Wu,J.Y., Guo,Z., Tsao,H.,
            De Luca,M., Catricala,C. and O'Toole,K.M.
  TITLE     A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence
            mechanism that limits replicative potential independent of telomere
            status
  JOURNAL   Mol. Cell. Biol. 22 (14), 5157-5172 (2002)
   PUBMED   12077343
  REMARK    GeneRIF: Keratinocytes engineered to express cdk4(R24C) and hTERT
            but not p53DD did not exhibit an extended life span.
REFERENCE   18 (bases 1 to 1474)
  AUTHORS   Morris,M., Hepburn,P. and Wynford-Thomas,D.
  TITLE     Sequential extension of proliferative lifespan in human fibroblasts
            induced by over-expression of CDK4 or 6 and loss of p53 function
  JOURNAL   Oncogene 21 (27), 4277-4288 (2002)
   PUBMED   12082615
  REMARK    GeneRIF: Sequential extension of proliferative lifespan in human
            fibroblasts is induced by over-expression of CDK4 or 6 and loss of
            p53 function.
REFERENCE   19 (bases 1 to 1474)
  AUTHORS   Korz,C., Pscherer,A., Benner,A., Mertens,D., Schaffner,C.,
            Leupolt,E., Dohner,H., Stilgenbauer,S. and Lichter,P.
  TITLE     Evidence for distinct pathomechanisms in B-cell chronic lymphocytic
            leukemia and mantle cell lymphoma by quantitative expression
            analysis of cell cycle and apoptosis-associated genes
  JOURNAL   Blood 99 (12), 4554-4561 (2002)
   PUBMED   12036888
REFERENCE   20 (bases 1 to 1474)
  AUTHORS   Kim,A.L., Athar,M., Bickers,D.R. and Gautier,J.
  TITLE     Ultraviolet-B-induced G1 arrest is mediated by downregulation of
            cyclin-dependent kinase 4 in transformed keratinocytes lacking
            functional p53
  JOURNAL   J. Invest. Dermatol. 118 (5), 818-824 (2002)
   PUBMED   11982759
  REMARK    GeneRIF: ultraviolet-B-induced cell cycle arrest in A431 cells is
            mediated by cyclin-dependent kinase 4 downregulation
REFERENCE   21 (bases 1 to 1474)
  AUTHORS   Haller,K., Wu,Y., Derow,E., Schmitt,I., Jeang,K.T. and Grassmann,R.
  TITLE     Physical interaction of human T-cell leukemia virus type 1 Tax with
            cyclin-dependent kinase 4 stimulates the phosphorylation of
            retinoblastoma protein
  JOURNAL   Mol. Cell. Biol. 22 (10), 3327-3338 (2002)
   PUBMED   11971966
  REMARK    GeneRIF: Results show that Tax directly interacts with CDK4.  The
            Tax/CDK complex represents an active holoenzyme which capably
            phosphorylates the Rb protein in vitro and is resistant to
            repression by the inhibitor p21(CIP).
REFERENCE   22 (bases 1 to 1474)
  AUTHORS   Leng,X., Noble,M., Adams,P.D., Qin,J. and Harper,J.W.
  TITLE     Reversal of growth suppression by p107 via direct phosphorylation
            by cyclin D1/cyclin-dependent kinase 4
  JOURNAL   Mol. Cell. Biol. 22 (7), 2242-2254 (2002)
   PUBMED   11884610
  REMARK    GeneRIF: Reversal of growth suppression by p107 via direct
            phosphorylation by cyclin D1/cyclin-dependent kinase 4
REFERENCE   23 (bases 1 to 1474)
  AUTHORS   Goldstein,A.M., Chidambaram,A., Halpern,A., Holly,E.A., Guerry
            IV,D., Sagebiel,R., Elder,D.E. and Tucker,M.A.
  TITLE     Rarity of CDK4 germline mutations in familial melanoma
  JOURNAL   Melanoma Res. 12 (1), 51-55 (2002)
   PUBMED   11828258
  REMARK    GeneRIF: Rarity of CDK4 germline mutations in familial melanoma.
REFERENCE   24 (bases 1 to 1474)
  AUTHORS   Nekhai,S., Shukla,R.R., Fernandez,A., Kumar,A. and Lamb,N.J.
  TITLE     Cell cycle-dependent stimulation of the HIV-1 promoter by
            Tat-associated CAK activator
  JOURNAL   Virology 266 (2), 246-256 (2000)
   PUBMED   10639311
REFERENCE   25 (bases 1 to 1474)
  AUTHORS   Harbour,J.W., Luo,R.X., Dei Santi,A., Postigo,A.A. and Dean,D.C.
  TITLE     Cdk phosphorylation triggers sequential intramolecular interactions
            that progressively block Rb functions as cells move through G1
  JOURNAL   Cell 98 (6), 859-869 (1999)
   PUBMED   10499802
REFERENCE   26 (bases 1 to 1474)
  AUTHORS   Zuo,L., Weger,J., Yang,Q., Goldstein,A.M., Tucker,M.A.,
            Walker,G.J., Hayward,N. and Dracopoli,N.C.
  TITLE     Germline mutations in the p16INK4a binding domain of CDK4 in
            familial melanoma
  JOURNAL   Nat. Genet. 12 (1), 97-99 (1996)
   PUBMED   8528263
REFERENCE   27 (bases 1 to 1474)
  AUTHORS   Wolfel,T., Hauer,M., Schneider,J., Serrano,M., Wolfel,C.,
            Klehmann-Hieb,E., De Plaen,E., Hankeln,T., Meyer zum
            Buschenfelde,K.H. and Beach,D.
  TITLE     A p16INK4a-insensitive CDK4 mutant targeted by cytolytic T
            lymphocytes in a human melanoma
  JOURNAL   Science 269 (5228), 1281-1284 (1995)
   PUBMED   7652577
REFERENCE   28 (bases 1 to 1474)
  AUTHORS   Mitchell,E.L., White,G.R., Santibanez-Koref,M.F., Varley,J.M. and
            Heighway,J.
  TITLE     Mapping of gene loci in the Q13-Q15 region of chromosome 12
  JOURNAL   Chromosome Res. 3 (4), 261-262 (1995)
   PUBMED   7606365
REFERENCE   29 (bases 1 to 1474)
  AUTHORS   Demetrick,D.J., Zhang,H. and Beach,D.H.
  TITLE     Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase
            genes
  JOURNAL   Cytogenet. Cell Genet. 66 (1), 72-74 (1994)
   PUBMED   8275715
REFERENCE   30 (bases 1 to 1474)
  AUTHORS   Serrano,M., Hannon,G.J. and Beach,D.
  TITLE     A new regulatory motif in cell-cycle control causing specific
            inhibition of cyclin D/CDK4
  JOURNAL   Nature 366 (6456), 704-707 (1993)
   PUBMED   8259215
REFERENCE   31 (bases 1 to 1474)
  AUTHORS   Kato,J., Matsushime,H., Hiebert,S.W., Ewen,M.E. and Sherr,C.J.
  TITLE     Direct binding of cyclin D to the retinoblastoma gene product (pRb)
            and pRb phosphorylation by the cyclin D-dependent kinase CDK4
  JOURNAL   Genes Dev. 7 (3), 331-342 (1993)
   PUBMED   8449399
REFERENCE   32 (bases 1 to 1474)
  AUTHORS   Hanks,S.K.
  TITLE     Homology probing: identification of cDNA clones encoding members of
            the protein-serine kinase family
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 84 (2), 388-392 (1987)
   PUBMED   2948189
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M14505.1 and BC003644.2.
            On or before Nov 4, 2002 this sequence version replaced
            gi:14249693, gi:4502734.
            
            Summary: The protein encoded by this gene is a member of the
            Ser/Thr protein kinase family. This protein is highly similar to
            the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a
            catalytic subunit of the protein kinase complex that is important
            for cell cycle G1 phase progression. The activity of this kinase is
            restricted to the G1-S phase, which is controlled by the regulatory
            subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase
            was shown to be responsible for the phosphorylation of
            retinoblastoma gene product (Rb). Mutations in this gene as well as
            in its related proteins including D-type cyclins, p16(INK4a) and Rb
            were all found to be associated with tumorigenesis of a variety of
            cancers. Multiple polyadenylation sites of this gene have been
            reported.
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q14"
     gene            1..1474
                     /gene="CDK4"
                     /note="synonyms: CMM3, PSK-J3, MGC14458"
                     /db_xref="GeneID:1019"
                     /db_xref="MIM:123829"
     CDS             228..1139
                     /gene="CDK4"
                     /EC_number="2.7.1.-"
                     /note="cell division kinase 4; melanoma cutaneous
                     malignant, 3;
                     go_function: ATP binding [goid 0005524] [evidence IEA];
                     go_function: transferase activity [goid 0016740] [evidence
                     IEA];
                     go_function: protein kinase activity [goid 0004672]
                     [evidence IEA];
                     go_function: cyclin-dependent protein kinase activity
                     [goid 0004693] [evidence TAS] [pmid 10499802];
                     go_process: cytokinesis [goid 0000910] [evidence IEA];
                     go_process: regulation of cell cycle [goid 0000074]
                     [evidence TAS] [pmid 7652577];
                     go_process: protein amino acid phosphorylation [goid
                     0006468] [evidence IEA];
                     go_process: G1/S transition of mitotic cell cycle [goid
                     0000082] [evidence TAS] [pmid 10499802]"
                     /codon_start=1
                     /product="cyclin-dependent kinase 4"
                     /protein_id="NP_000066.1"
                     /db_xref="GI:4502735"
                     /db_xref="GeneID:1019"
                     /db_xref="MIM:123829"
                     /translation="MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGG
                     GGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRT
                     YLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFG
                     LARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNS
                     EADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEML
                     TFNPHKRISAFRALQHSYLHKDEGNPE"
     misc_feature    492..841
                     /gene="CDK4"
                     /note="Region: the region absent in variant 2"
     polyA_site      1413
                     /gene="CDK4"
                     /evidence=experimental
     polyA_site      1420
                     /gene="CDK4"
                     /evidence=experimental
     polyA_site      1423
                     /gene="CDK4"
                     /evidence=experimental
     polyA_site      1428
                     /gene="CDK4"
                     /evidence=experimental
     polyA_site      1442
                     /gene="CDK4"
ORIGIN      
        1 agccctccca gtttccgcgc gcctctttgg cagctggtca catggtgagg gtgggggtga
       61 gggggcctct ctagcttgcg gcctgtgtct atggtcgggc cctctgcgtc cagctgctcc
      121 ggaccgagct cgggtgtatg gggccgtagg aaccggctcc ggggccccga taacgggccg
      181 cccccacagc accccgggct ggcgtgaggg tctcccttga tctgagaatg gctacctctc
      241 gatatgagcc agtggctgaa attggtgtcg gtgcctatgg gacagtgtac aaggcccgtg
      301 atccccacag tggccacttt gtggccctca agagtgtgag agtccccaat ggaggaggag
      361 gtggaggagg ccttcccatc agcacagttc gtgaggtggc tttactgagg cgactggagg
      421 cttttgagca tcccaatgtt gtccggctga tggacgtctg tgccacatcc cgaactgacc
      481 gggagatcaa ggtaaccctg gtgtttgagc atgtagacca ggacctaagg acatatctgg
      541 acaaggcacc cccaccaggc ttgccagccg aaacgatcaa ggatctgatg cgccagtttc
      601 taagaggcct agatttcctt catgccaatt gcatcgttca ccgagatctg aagccagaga
      661 acattctggt gacaagtggt ggaacagtca agctggctga ctttggcctg gccagaatct
      721 acagctacca gatggcactt acacccgtgg ttgttacact ctggtaccga gctcccgaag
      781 ttcttctgca gtccacatat gcaacacctg tggacatgtg gagtgttggc tgtatctttg
      841 cagagatgtt tcgtcgaaag cctctcttct gtggaaactc tgaagccgac cagttgggca
      901 aaatctttga cctgattggg ctgcctccag aggatgactg gcctcgagat gtatccctgc
      961 cccgtggagc ctttcccccc agagggcccc gcccagtgca gtcggtggta cctgagatgg
     1021 aggagtcggg agcacagctg ctgctggaaa tgctgacttt taacccacac aagcgaatct
     1081 ctgcctttcg agctctgcag cactcttatc tacataagga tgaaggtaat ccggagtgag
     1141 caatggagtg gctgccatgg aaggaagaaa agctgccatt tcccttctgg acactgagag
     1201 ggcaatcttt gcctttatct ctgaggctat ggagggtcct cctccatctt tctacagaga
     1261 ttactttgct gccttaatga cattcccctc ccacctctcc ttttgaggct tctccttctc
     1321 cttcccattt ctctacacta aggggtatgt tccctcttgt ccctttccct acctttatat
     1381 ttggggtcct tttttataca ggaaaaacaa aacaaagaaa taatggtctt tttttttttt
     1441 ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//
