Slides: [Color, 1 per page]
[to print (4 per page)]
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Exercise 1: Browsing the Aging, Dementia and Traumatic Brain on aging.brain-map.org
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Exercise 2: Go to Tabula Muris, scroll down to "Visualization".
Select FACS and Brain Non-Myeloid and look one at a time the list of genes in this table. Write where each gene is expressed. What "marker genes" could you use to identify each type of cell?
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Exercise 3: Find which brain cells express GAD1 and GFAP by looking at immune staining of those proteins in the brain: proteinatlas.org
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Exercise 4: Questions on Tay-Sachs disease:
4a: Which gene is responsible for the disease? Go to OMIM and search for "Tay-Sachs disease"
4b: How does the mutation of this gene result in Tay-Sachs disease? On the same OMIM page, look under "Biochemical features".
4c: What mutations in this gene cause Tay-Sachs disease? On the same OMIM page, look under "Molecular Genetics". For a more complete list, go to the UCSC Genome Browser (human hg38) and search for the gene. Under "Phenotype and literature", set "ClinVar Variants" to "Pack". The red variants are pathogenic.
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| Exercise 2 solution
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